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Players will walk through cities as rain splashes down onto the streets with puddles reflecting the glow of neon signs, streetlights, and other details of urban life. #Max payne 2 the fall of max payne cast professionalThe new graphic engine allows for motion-captured movement, facial animation from a cast of professional actors, lip-synching, new lighting techniques, a rag doll physics model using the licensed Havok development kit, and redesigned 3D characters. Things are never easy for Payne, however, as Sax just so happens to be a murder suspect who leads him down a dark path from which he may be unable to walk away from. The Fall of Max Payne chronicles the title character's romance with a sultry siren named Mona Sax. The stylized third-person shooter, which initially turned heads with its combination of gritty environments, film noir style, and use of bullet-time effects for dramatic shootouts, returns with an enhanced graphic engine and completely new storyline.
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We have to use other tools to manage our Windows and Android devices. #JAMF PRO SHARED IPAD MAC#We'd that option available for Mac." "It will be very helpful if we could restore users after they have been removed from Jamf because I have some cases of deleting a computer before unlocking it." "The on-going improvements & innovation that Jamf provides over time is something that needs to be maintained, as it is what is needed to fulfill a business & team need at the right time." "Jamf Pro currently is only used on Mac computers and could improve by having compatibility with other operating systems. A mobile app to complement the web-based version, to perform specific tasks, would be nice to have - a good example of what I mean is the "Pulseway" MDM for Windows, they have a very useful and user-friendly mobile app." "For Windows, we have a reset option. "I would like to see either a report section or an integration with a new reporting module to allow us to pull real metrics from the system easily." "The product is pretty good as-is, but if anything could be improved I would say the UI could perhaps be a bit simpler. This allows users to have an out of box experience." There's no jumping around multiple UI's to do your overall management." "It is a very stable and scalable cloud-only solution." "The most valuable features are the ones that make sure that the deployment is of a standard operating system and the Zero Touch deployment, which is very useful. That is the most important part." "It works pretty well for us." "The most valuable feature is the UEM capabilities." "The solution is easy to use and it has good performance." "I would say the biggest benefit is the single-pane view. I don't need to worry about the network connectivity between the offices. We have installed Intune to control the user's environment minimizing the chances of any hacking." "I can reach devices or computers over the internet. "By using Microsoft Intune we can control which websites the users can go to and it provides a secure environment for our employees using their laptops that are having access from home. However, it is more expensive than Microsoft Intune. Its ease of deployment, its solid set of features, and its service and support all top Microsoft Intune’s offerings.
![]() ![]() ![]() The truth is that festivals are not an absolute priority for all producers, which means that some films simply don’t show up anywhere, or only at smaller festivals that are considered useful for distribution reasons.” There is no need to hide the fact it is getting more and more difficult, but it is the same for everyone. “I am pretty excited about the lineup,” he says. Its opening film is the British-produced mountaineering thriller Everest, featuring Anglo-American glamour in the shape of Jake Gyllenhaal, Keira Knightley and Josh Brolin its competition strand has an impressive list of international auteurs, including Tom Hooper (The Danish Girl), Alexander Sokurov (Francofonia), Luca Guadagnino (A Bigger Splash) and Charlie Kaufman (Anomalisa) and a number of authentic coups, including the world premiere screening of Black Mass, the much-hyped gangster film featuring Johnny Depp as James “Whitey” Bulger, and a first look at Beasts of No Nation, the African-set war thriller that represents Netflix’s most serious shot yet across Hollywood’s bows.Īlberto Barbera, the festival director, and the man primarily responsible for securing the festival programme, is quietly confident. As it gears up for its 11-day extravaganza on the Lido, the long, narrow island to the south-east of the ancient city of Venice, the film festival is looking forward to better times. ![]() ![]() ![]() Please see the suggestions below for tips on optimizing your WB on phosphorylated proteins.ġ. ![]() After washing with tap water to remove residual fixative, dry at room temperature.Ĭarrying out western blots on phosphorylated proteins can require further optimization and a few unique conditions. After development, the film should be immediately immersed in the fixing solution to a transparent film. Take out the film at a red light, open the film clip, put the film on the membrane, remove the film clip, adjust the exposure time based on the strength of the signal, remember that an over-exposed film is not suitable for analysis.Īfter the exposure is complete, remove the film, quickly immerse in developing solution, terminate development. Ensure the membrane surface proteins have sufficient contact with the mixture. In darkroom, load the developer and fixative respectively into plastic trays mixing the two reagents, volume of A and B, in a centrifuge tube. Incubate with the secondary antibody, the same as step (4), dilution with TBST, 37℃ shaking incubation 1 ~ 3 h.Ĭhemiluminescence, developing and fixing, for HRP-conjugated antibodies: ECL are the traditional kits used. Then load the membrane with a ziplock bag and fixed lamination machine, add the antibody solution to the ziplock, and make sure the membranes are incubated with sufficient volume of antibody.ĥ) 37℃ shaking incubation 1 ~ 3 h (according to the antibody titer and affinity ), or 4 ℃ overnight incubation, TBST wash on the shaker at room temperature for 3 times, each time 5 ~ 10 min.Ħ). According to the recommended antibody dilution, dilute antibody with TBST (or 1% to 3% non-fat milk). Blocking at 4 ℃ with shaker shaking overnight, or 37 ℃ blocking for 2 hours. Block the membrane generally with 5% non-fat milk, or 3%~5% BSA. Then rinse off the dye with distilled water.ģ). After the transfer, use Ponceau dye staining about 2 ~ 5min to check the transfer is successful or not. ![]() (according to the instructions recommended by trans-blot system.)Ģ). Transfer the protein to the membrane from the gel. Transfer of proteins and staining (Western blotting)ġ). Subject to electrophoresis according to the instructions recommended by electrophoresis method, Terminate the gel electrophoresis when bromophenol blue runs out the gel. Load samples and run the gel, each hole sample volume 20-40μg protein, pay attention when spotting the sample, do not overflow into adjacent wells which will cause gum poked holes and cross-contamination.ĥ). 4% Stacking gel, select pre-stained protein Marker Proteins in size, to help distinguish the size and protein electrophoresis tracer.Ĥ). Prepare PAGE gels, choose different concentrations of separating gel depending on the molecular weight of the protein:ģ). Clean the glass and set up the electrophoresis system.Ģ). To denature, use a loading buffer with the anionic denaturing detergent sodium dodecyl sulfate (SDS), and boil the mixture at 95-100☌ for 5 minutes.ġ). Preparation of samples for loading into gels Bovine serum albumin (BSA) is a frequently-used protein standard. Use PAGE gels, a Bradford assay, a Lowry assay or a BCA assay. Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice discard the pellet. The RIPA (with 1mM pmsf ) should be ice-cold prior to homogenization.Ĭut the tissue into small pieces, For a ~20 mg piece of tissue, add ~250 μl lysis buffer rapidly to the tube, homogenize with an electric homogenizer (or a glass homogenizer) until fully lysed.Ĭentrifuge for 5 min at 10000~14000g at 4☌ in a microcentrifuge. Pmsf (Phenylmethanesulfonyl fluoride) is the protease inhibitors we always use.ĭissect the tissue of interest with clean tools, on ice preferably, and as quickly as possible to prevent degradation by proteases. These events can be slowed down tremendously if samples are kept on ice or at 4☌ at all times and appropriate inhibitors are added fresh to the lysis buffer. ![]() We use RIPA buffer (beyotime P0013B) for whole cell extracts and membrane-bound proteins.Īs soon as lysis occurs, proteolysis, dephosphorylation and denaturation begin. This solubilizes the proteins so they can migrate individually through a separating gel. To prepare samples for running on a gel, cells and tissues need to be lysed to release the proteins of interest. ![]() ![]() Its just its more noticeable now as there are perhaps more of them and while they were once poor they fell into the privileged society so just by being white they got to do much as they pleased and with that mindset felt rich. Now 21 years after the first democratic elections SA has returned to being a primarily one-party state, and while all citizens have the vote the minority group in SA is no longer the Africans, or even the Indo-Asians, nor those of mixed race people, but rather the Caucasian group that find themselves in a state of poverty that they haven’t felt since the Boer War.īut its an unfortunate truth that there were many of these same families in poverty under the National Party as there are now under the governance of the ANC. The NP had lauded it over South Africa for 18 years before the border wars started and only 12 years before the ANC and the PAC joined forces after the Sharpeville massacre, and it was 41 years later that the white regime fell in SA almost at the same time as communism failed in the USSR. The NP represented everything I disagreed with (other than FW De Klerk emancipating Mandela), but while I agreed with the ANC regarding the need for total democracy I did not agree with the methodology that the ANC supporters had used on its opponents. So the true idea of democracy was thrown out of the window as there could be no truly representational government when one group is almost enforced to vote against what they really believed in to achieve a political harmony and smooth transition. The first time I voted in South Africa, was like every black South African……in the 1994 elections…….and I couldn’t vote for policies or even on popularity I had to choose to vote for a party I dislike immensely among about 20+ different parties that had all come out to have their policies voted on in the first really democratic election.īut a vote for any of those other parties, other than the ANC or the National Party (NP) would have meant a non-coalition government and SA needed something more tempered at the beginning of the process, and only solid voting numbers for one or two parties – the ANC literally had it in the bag with more than 50% of the voters voting not only for democracy but showering support and loyalty to a man that had spent 27 years in jail trying to achieve that aim. In South Africa in the 1980s, regardless of age or gender if you had been in the country longer than 5 years you were automatically naturalised – this was to ensure more young men could be drafted into the military services to keep the northern SA borders safe from both the “Swart gevaar” and the “Commies”. ![]() In most countries immigrants can choose the right to become a citizen and in some cases hold dual citizenship for their new and home countries. I left the UK in the 1960s when the cry of the British was “look out the Paki’s are coming”….and….”there goes the neighbourhood” and arrived in South Africa to cries of “bewaar die Swart gevaar” (loosely translated meaning – warning, here comes the Black danger) echoes of news reports with comments like “beware of the Reds” or the “Commies are coming”. ![]() But this enjoyment is set in stark contrast to how many of the indigenous people of the country you have just immigrated to are treated. However you can usually own property, get a job and enjoy all the other freedoms that said country will provide as a ‘human’ right. While your parents may be of voting age most countries require that you become a citizen of the country before you or your children get the voting right. Being an immigrant in a new country as a child and non-voter there is very little one has to say about the governance of the country that you have just immigrated to. ![]() ![]() ![]() This war actually was quite traumatizing to famed primatologist Jane Goodall. The Gombe Chimpanzee was a conflict that took place from 1974-1978 with mass casualties on both sides. This is the first interspecies primate war ever seen but not the first Chimpanzee war ever recorded. Gorillas are gentle giants that spend their day foraging and lounging, while chimps have been known to engage in advanced almost military-like conflict with other groups. Primatologists have always commented that Chimps are much more warlike than Gorillas. It's hard to imagine that the larger Gorillas would be overpowered by the Chimps but the Chimps vastly outnumbered the Gorillas. The researchers commented that the two species were sometimes playful and relaxed toward each other but one day the chimpanzees went "Apeshit" and decided to go on a murder rampage on an outnumbered group of gorillas. The belt stretches from southern Senegal through central Africa to western Tanzania.On July 22nd researchers in the Loango National Park observed a violent encounter between a troop of Chimpanzees and a band of Gorillas. Today conservation figures put the number of chimpanzees in what is known as the chimpanzee belt at about 300,000. The chimpanzee attacks in Kasooha sound familiar across much of equatorial Africa where the primates once roamed freely in their millions. Conservationists fear this could destroy tourism activities that directly depend on wild ape populations like gorilla, baboons and chimpanzee. The primary threats to chimpanzees in recent years have been habitat destruction (deforestation), logging, hunting and disease.Īll these are blamed on the rapid human population growth. Ugandan conservationists estimate that Uganda has about 5000 chimpanzee in the wild, mainly in western Uganda. “The forest used to host chimps, they could sleep and also get their food there but now chimps are on the constant search for new places to stay.” “As communities we are paying the price for the encroachers like Kinyara sugar Works who cleared the 40 sq km Rwebitela forest reserve for sugar cane farming,” he says. The incident happened 10 years ago in 2009 but Mugurusi recalls it like it happened yesterday.īaguma Moses, who neighbours Mugurusi, blames the chimpanzee behaviour towards them to Kinyara Sugar Works, which he says, destroyed a forest that was home to most of the chimps. “They simply said we had disturbed the chimpanzees and that the penalty for killing a chimpanzee ranges between 10 to 15 years in prison, so we had to give up,” Mugurusi recalls. But Mugurusi says they did not receive much help. The baby’s body was taken to the nearby Masindi Hospital for a postmortem to confirm cause of death and evidence to seek justice from the national conservation agency-the Uganda Wildlife Authority. Mugurusi says his grandchild died instantly. When the noise intensified, the chimp dropped the baby. ![]() Armed with sticks, clubs and pangas, they mounted a search but the chimp climbed a tree with the baby. She raised an alarm that attracted neighbours in nearby gardens. ![]() Mugurusi, 76, says her daughter had no chance of rescuing the baby on her own. It jumped from its hideout, grabbed the baby, and dashed into the bush. Unknown to her, a chimpanzee in a nearby thicket was watching, and monitoring her movements. She placed the seven-month-old baby under a shed as she started working. Mugurusi’s daughter had gone to her garden on the forest edge. Issa Isingoma Mugurusi belongs to another group – of indirect sufferers of chimp attacks. Unlike most of her peers who go to school, she has not gone to school. She was lucky to live but the ape bit her and left her with permanent memory loss. She was only four at the time and was home alone when the chimpanzee pounced. Mariam Mirungi, 14, tells the story of how she fought a chimpanzee. They tell stories of how they have wrestled a chimpanzee and survived. Many residents have horror stories involving the chimps. They eat their crops, attack children and women, and make life hard for humans. The large apes are terrorising residents. Kampala, Uganda | JIMMY SIYA| Kasooha central forest reserve in Kasongoire Parish in Masindi District, in the Bunyoro region of western Uganda is chimpanzee country. Community in Bunyoro lives under terror of primates. ![]() ![]() Instead of the old “towel method” are parents used, roll the pumpkin cake inside the parchment paper it bakes on. Plus, I’ve got a little trick that makes this recipe mess-free. Every bite is like a little slice of heaven.Īlthough it may look a little intimidating, this pumpkin roll is surprisingly easy to make! Well, what are you waiting for, whip on your apron, and lets get recipe hunting: 1. Plus, most of the recipes don’t call for any odd ingredients you can’t find nearby. The taste of fall with a delicious cream cheese filling. Better yet, these recipes will make for diabetes-friendly desserts and food - sweet tooth satisfying without the blood-sugar level spike. Pumpkin Rollįor me, the holidays just aren’t complete without a pumpkin roll. While this dump cake is fantastic on its own, you also can’t go wrong with a dollop of vanilla ice cream on top. It’s so simple that there is absolutely no way to mess it up. This pumpkin dump cake uses a simple mixture of pumpkin, eggs, milk, sugar, and pumpkin pie spice for the topping, and a yellow cake mix for the cake. Too busy to make something fancy for Thanksgiving? Pumpkin dump cake is here to save the day.Ī dump cake is an incredibly easy dessert made by dumping cans of fruit in a pan and sprinkling cake mix over it.Īll that’s left is to pop it in the oven, and you’ll have the most decadent, fruity dessert! This warm bowl of comfort is packed with fresh apples, warm spices, and a crispy oatmeal crumb topping. Especially if you top it off with a scoop of vanilla ice cream. This old-fashioned apple crisp makes the perfect Thanksgiving dessert. Looking for something warm and comforting that feeds your soul and makes the house smell heavenly all day long? Oh my! There is no way anyone can resist this pumpkin pie.Īlso freezes well. To top it off, they even give you a recipe for a delicious frosting. Then guess what You have a delicious cake on your hands. ![]() All you need is a boxed cake mix, some pineapple with the juice, some eggs, and oil. Velvety smooth pumpkin pie filling seasoned with autumn spices, resting on a flaky crust, topped with a dollop of whipped cream. Pineapple Sunshine Cake This cake not only looks delicious, but it is so simple to make. If you love peaches, be sure to make my Perfect Peach Pie 20. ![]() No Thanksgiving dinner is complete without homemade pumpkin pie! Top it off with some whipped topping for the ultimate holiday treat. Peaches and Cream Pie is a no-bake dessert that is made with a shortbread cookie crust, sweet peach filling, a cream cheese layer, and topped with a homemade whipped topping. 24 Best Thanksgiving Dessert Recipe Collection.Snickers Chocolate Peanut Butter Pumpkin Fudge ![]() |
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